Abstract: Enterobacter sakazakii has been implicated in a several form of neonatal meningitis with a high mortality rate. In the present study, the speciesspecific PCR and oligonucleotide array assays were developed to detect the 16S–23S rDNA...
Estimation of bacterial species phylogeny through oligonucleotide frequency distances
Classification of bacteria is mainly based on sequence comparisons of certain homologous genes such as 16S rRNA. Recently there are challenges to classify bacteria using oligonucleotide frequency pattern of nonhomologous sequences. However,...
Identification of Bacillus spp., Escherichia coli, Salmonella spp., Staphylococcus spp. and Vibrio spp. with 16S ribosomal DNA-based oligonucleotide array hybridization
Rapid identification of the genus and species of bacteria in foods and clinical specimens is important. In this report, DNA sequences of bacterial 16S rDNA were used to develop the oligonucleotide array for the identification of bacterial strains...
Design and application of an oligonucleotide microarray for the investigation of compost microbial communities
A microarray consisting of oligonucleotide probes targeting variable regions of the 16S rRNA gene was designed and tested for the investigation of microbial communities in compost. Probes were designed for microorganisms that have been previously...
A low-density oligonucleotide array study for parallel detection of harmful algal species using hybridization of consensus PCR products of LSU rDNA D2 domain
Abstract: A low-density oligonucleotide array approach based on the hybridization of consensus PCR products of LSU rDNA was developed in order to simultaneously detect various harmful algae. A set of oligonucleotide probes for the hybridization...
Application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections
Abstract: A rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. Nylon membrane was used as the array support. A mutation region of the 23S...
Design and application of group-specific oligonucleotide probes for detecting and monitoring mouse clostridia
Abstract: Clostridia dominate the rodent intestinal bacterial community and play an important role in physiological functions of the host. However, their ecology and diversity are still unclear. In our previous report,...
Fast Impurity Profiling of Synthetic Oligonucleotides
Moritz Wagner, Zoltan Timar, Edgar Naegele and Gordon Ross, Agilent Technologies, Waldbronn, Germany.
IntroductionRecognition of DNA's...
Oligonucleotide primers, probes and molecular methods for the environmental monitoring of methanogenic archae
Summary: For the identification and quantification of methanogenic archaea (methanogens) in environmental samples, various oligonucleotide probes/primers targeting phylogenetic markers of methanogens, such as 16S rRNA, 16S rRNA gene and...
Portable System for Microbial Sample Preparation and Oligonucleotide Microarray Analysis
Abstract: We have developed a three-component system for microbial identification that consists of (i) a universal syringe-operated silica minicolumn for successive DNA and RNA isolation, fractionation,...
Oligonucleotide Analysis
Oligonucleotides are polymeric sequences of nucleotides (RNA, DNA, and their analogs). Oligonucleotides are utilized for basic molecular research employing primers and siRNAs, health diagnostics and pharmacogenomic studies using microarrays...
Oligonucleotide
Synonymous of oligonucleotid are: oligomer, oligo, N-mer (where N is a number, e.g. 22-mer)
Oligonucleotides are short chains of synthetic deoxyribose nucleic acid (DNA). They are usually around 20-30 nucleotides and can be formed by bond cleavage...
DNA-RNA-Oligonucleotide
The deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the unique biopolymers responsible for storage and transfer of genetic information. They are ubiquitous in all living systems on earth.
DNA occurs in nature...
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