Oligonucleotides are polymeric sequences of nucleotides (RNA, DNA, and their analogs). Oligonucleotides are utilized for basic molecular research employing primers and siRNAs, health diagnostics and pharmacogenomic studies using microarrays and fluorogenic probes, and pharmaceutical therapeutics utilizing antisense oligos, aptamers, and miRNAs. They are often modified to enhance their mechanistic functionality, hybridization specificity and efficiency, or nuclease resistance.
Oligonucleotides are produced by automated solid-phase synthetic processes. Typical lengths range from 20 to 80 nucleotides, and synthesis scales vary from nanograms to kilograms, depending on the application. Although the oligo synthesis process is highly efficient, sequence truncated oligos and other process-related modifications and impurities are often artifacts of synthesis. It's important to analyze synthetic products to determine if purity is sufficient. Intermediate and final analysis of the synthesis products are also useful for Quality by Design studies and process control.
The solid-phase chemical synthesis process and molecular diversity of oligos have made analytical characterization highly challenging. The ACQUITY UPLC System brings a new level of chromatographic performance, resolution, sensitivity, and throughput that enables laboratories to be more productive.
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