1. INTRODUCTION
1.1 Acute retinal necrosis (ARN)
ARN syndrome is a well-known clinical entity that was first described in 1971 (Urayama et al., 1971). ARN is a type of retinitis that affects both healthy and immunocompromised patients (Silverstein...
Computational Methods for Rational Oligonucleotide PCR Primer Design and Analysis: Two Scenarios Using GCG¥’s SeqLab. By Steven M. Thomson (stevet@bio.fsu.edu)
IntroductionThe Polymerase Chain Reaction, PCR, developed at Cetus Corporation by Kary Mullis in the mid ‘80’s (Saiki, et al., 1988), for which he won the Nobel Prize, and patented by Hoffman La Roche and Perkins-Elmer Corporation, has revolutionized...
Antisense Oligonucleotide
This intriguing area started in 1970s targeting viruses, and then moved to humans to target DNA. The basic idea of this novel therapeutic approach is to synthesize a short oligonucleotide complementary to a sequence of a certain gene. Hybridization...
Genomic DNA Hyb Protocol for Oligonucleotide Array (Indirect labeling)
Shear gDNA (Hydroshear, Gene Machines) speed code 4, 30 shearing cycles, wash 3x, 2x, 5x with acid, base, TE washes as recommend by the manufacturer. (Digesting with a random four cutter is an option but may cleave in the oligo sequence for...
A Research on Arginine-Rich Peptides Conjugated Oligonucleotide Targeting to Telomerase
A Research on Arginine-Rich Peptides Conjugated Oligonucleotide Targeting to Telomerase Yuefeng Peng , Changpo Chen , Lihe Zhang National Research Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences and...
8th Annual Meeting of the Oligonucleotide Therapeutics Society
Join in October 28-31 in Boston, Massachusetts for the
8th Annual Meeting of the Oligonucleotide Therapeutics Society!Join leading experts in the field during this three day meeting, which will feature the latest developments from various...
FLUORESCENCE IN SITU HYBRIDIZATION USING 16S rRNA-TARGETED OLIGONUCLEOTIDE DETERMINES OF THE IDENTIFIED SYNTROPHIC SUBSTRATE OXIDIZING ANAEROBES
Fluorescence in situ hybridization (FISH) was employed to elucidate of mesophilic (35°C) and thermophilic (55°C) syntrophic substrate oxidizing anaerobes, individually. The whole microbe cell was visualized in a wet amount of each enrichment...
Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria
PCR primer sets for the 16S rRNA gene of six phylogenetic groups of sulfate-reducing bacteria (SRB) were designed. Their application in conjunction...
Using next generation sequencing to identify yellow fever virus in Uganda
In October and November 2010, hospitals in northern Uganda reported patients with suspected hemorrhagic fevers. Initial tests for Ebola viruses, Marburg virus, Rift Valley fever virus, and Crimean Congo hemorrhagic fever virus were negative....
Elaboration of silica colloid/polymer hybrid support for oligonucleotide synthesis
One way to increase the sensitivity of DNA diagnostic assays developed on microarrays is to improve the solid phase that allows the extraction of the target from a biological sample, before detection. Two parameters are influencing the performances...
Universal ribotyping method using a chemically labelled oligonucleotide probe mixture
Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5)...
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